Human Alk-Smase (Alkaline Sphingomyelinase) CLIA Kit (HUES00225)
- SKU:
- HUES00225
- Product Type:
- ELISA Kit
- ELISA Type:
- CLIA Kit
- Size:
- 96 Assays
- Sensitivity:
- 46.88pg/mL
- Range:
- 78.13-5000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- ENPP7
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Metabolism
Description
Product Name: | Human Alk-Smase (Alkaline Sphingomyelinase) CLIA Kit |
SKU: | HUES00225 |
Target: | Human Alk-Smase (Alkaline Sphingomyelinase) |
Size: | 96T |
Assay type: | Sandwich |
Assay time: | 4.5h |
Sensitivity: | 46.88 pg/mL |
Detection range: | 78.13-5000 pg/mL |
Kit component: |
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This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Alk-Smase. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Alk-Smase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Alk-Smase, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Alk-Smase. You can calculate the concentration of Human Alk-Smase in the samples by comparing the RLU value of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | Tissue homogenates; 100μL |
Reproducibility: | Both intra-CV and inter-CV are < 15%. |
Application: | This CLIA kit applies to the in vitro quantitative determination of Human Alk-Smase concentrations in Tissue homogenates. |
Specificity: | This kit recognizes Human Alk-Smase in samples. No significant cross-reactivity or interference between Human Alk-Smase and analogues was observed. |