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Human Aldose Reductase Like Protein 1 (ARL1) ELISA Kit

SKU:
HUDL00243
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O60218
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AKR1B10,AKR1B12,HIS,HSI,ALDRLn,Aldo-Keto Reductase Family 1,Member B10,Aldose Reductase-Related Protein,Small Intestine Reductase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

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Human Aldose Reductase Like Protein 1 (ARL1) ELISA Kit

The Human Aldose Reductase-Like Protein 1 (ARL1) ELISA Kit is specifically designed for the precise detection of ARL1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ARL1 is a protein that plays a critical role in cellular processes such as vesicular trafficking and membrane organization. Dysregulation of ARL1 has been implicated in various diseases, including cancer, metabolic disorders, and neurological conditions.

Therefore, the ARL1 ELISA Kit is a valuable tool for researchers studying these conditions and exploring potential therapeutic interventions.Overall, the Human ARL1 ELISA Kit is a reliable and efficient solution for quantifying ARL1 levels in human samples, providing valuable insights into the mechanisms underlying disease pathology and potential treatment strategies.

Product Name:Human Aldose Reductase Like Protein 1 (ARL1) ELISA Kit
Product Code:HUDL00243
Size:96 Assays
Target:ARL1
Synonyms:AKR1B10, AKR1B12, HIS, HSI, ALDRLn, Aldo-Keto Reductase Family 1,Member B10, Aldose Reductase-Related Protein, Small Intestine Reductase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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