Human ALDOA (Aldolase A, Fructose Bisphosphate) ELISA Kit
The Aldolase A, Fructose Bisphosphate (ALDOA) ELISA Kit has been meticulously developed for the specific and accurate quantitation of human ALDOA in diverse biological samples.
ALDOA, a critical enzyme in glucose metabolism, catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Its role in glycolysis underscores its involvement in fundamental cellular processes like energy production and biosynthesis. Accurate measurement of ALDOA levels enables a deeper understanding of its functions in various physiological and pathological conditions, including metabolic disorders, cancer, and neurodegenerative diseases. Our ALDOA ELISA Kit ensures exceptional sensitivity and specificity, guaranteeing precise and reproducible results. Manufactured under strict quality controls for robust performance, this kit offers ease of use, making it suitable for a wide range of research and clinical applications. Count on Assay Genie's ALDOA ELISA Kit for reliable quantification of this essential biomarker in your investigations and studies.
Product Name:
Human ALDOA (Aldolase A, Fructose Bisphosphate) ELISA Kit
SKU:
AEES00069
Target:
Human ALDOA (Aldolase A, Fructose Bisphosphate)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ALDOA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALDOA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALDOA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ALDOA. You can calculate the concentration of Human ALDOA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
94-105
89-102
Average (%)
98
99
95
1:4
Range (%)
90-100
95-104
94-108
Average (%)
94
101
100
1:8
Range (%)
89-99
98-105
90-102
Average (%)
95
101
98
1:16
Range (%)
92-101
100-110
95-107
Average (%)
98
105
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-98
95
EDTA plasma (n=5)
94-103
98
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
2.48
8.46
0.85
2.14
9.79
Standard deviation
0.06
0.12
0.38
0.04
0.1
0.47
C V (%)
6.12
4.84
4.49
4.71
4.67
4.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ALDOA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ALDOA in samples. No significant cross-reactivity or interference between Human ALDOA and analogues was observed.
