Human ALCAM (Activated Leukocyte Cell Adhesion Molecule) ELISA Kit
The Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit is meticulously crafted to accurately quantify human ALCAM levels in a diverse range of biological samples. ALCAM, a transmembrane receptor protein, plays a pivotal role in cell-cell interactions, including adhesive and signaling processes crucial for immune responses, inflammation, angiogenesis, and nervous system development. Dysregulation of ALCAM expression has been associated with various diseases, making precise measurement imperative for understanding pathophysiological mechanisms and potential therapeutic interventions.
Our ALCAM ELISA Kit provides exceptional sensitivity and specificity, ensuring precise and reproducible results essential for your research and clinical investigations. With stringent quality control measures in place, this kit guarantees robust performance and ease of use, making it an optimal choice for a wide array of applications. Rely on Assay Genie's ALCAM ELISA Kit for reliable and accurate quantification of this fundamental biomarker in your scientific studies and endeavors.
Product Name:
Human ALCAM (Activated Leukocyte Cell Adhesion Molecule) ELISA Kit
SKU:
AEES00068
Target:
Human ALCAM (Activated Leukocyte Cell Adhesion Molecule)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ALCAM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ALCAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ALCAM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ALCAM. You can calculate the concentration of Human ALCAM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-94
92-105
89-98
Average (%)
91
97
95
1:4
Range (%)
90-106
98-109
93-102
Average (%)
98
105
98
1:8
Range (%)
96-106
96-108
90-102
Average (%)
102
101
94
1:16
Range (%)
92-103
88-96
99-110
Average (%)
97
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
93
EDTA plasma (n=5)
97-107
100
Cell culture media (n=5)
93-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
90.04
244.75
932.15
99.7
213.12
972.34
Standard deviation
4.94
12.07
43.16
5.12
10.17
42.49
C V (%)
5.49
4.93
4.63
5.14
4.77
4.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ALCAM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ALCAM in samples. No significant cross-reactivity or interference between Human ALCAM and analogues was observed.
