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Human Alanine Aminotransferase (ALT) ELISA Kit

SKU:
HUDL00152
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P24298
Sensitivity:
0.132ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
GPT,ALAT,SGPT,AAT1,GPT1,Serum Glutamic Pyruvic Transaminase,Alanine Transaminase,Glutamate pyruvate transaminase 1,Glutamic--alanine transaminase 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Alanine Aminotransferase (ALT) ELISA Kit

The Human Alanine Aminotransferase (ALT) ELISA Kit is a reliable tool for the quantitative measurement of ALT levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Alanine aminotransferase (ALT) is a key enzyme involved in amino acid metabolism, specifically the breakdown of alanine. Elevations in ALT levels in the blood are commonly associated with liver damage or disease, making this kit valuable for monitoring liver health and diagnosing liver conditions.

By using the Human ALT ELISA Kit, researchers can gain insights into liver function, monitor disease progression, and evaluate the effectiveness of potential treatments. With its high-performance capabilities, this kit is an essential tool for studying liver physiology and pathology.

Product Name:Human Alanine Aminotransferase (ALT) ELISA Kit
Product Code:HUDL00152
Size:96 Assays
Target:ALT
Synonyms:GPT, ALAT, SGPT, AAT1, GPT1, Serum Glutamic Pyruvic Transaminase, Alanine Transaminase, Glutamate pyruvate transaminase 1, Glutamic--alanine transaminase 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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