Human AICDA Recombinant Protein (RPPB1367)
- SKU:
- RPPB1367
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9GZX7
- Research Area:
- Enzymes
Description
| Product Name: | Human AICDA Recombinant Protein |
| Product Code: | RPPB1367 |
| Size: | 20µg |
| Species: | Human |
| Target: | AICDA |
| Synonyms: | Single-stranded DNA cytosine deaminase, Activation-induced cytidine deaminase, Cytidine aminohydrolase, AICDA, AID, ARP2, CDA2, HIGM2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The AICDA solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MDSLLMNRRK FLYQFKNVRW AKGRRETYLC YVVKRRDSAT SFSLDFGYLR NKNGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVADFLRG NPNLSLRIFT ARLYFCEDRK AEPEGLRRLH RAGVQIAIMT FKDYFYCWNT FVENHERTFKAWEGLHENSV RLSRQLRRIL LPLYEVDDLR DAFRTLGL |
Activation-Induced Cytidine Deaminase (AICDA) belongs to the the cytidine deaminase family. AICDA is involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. AICDA is necessary for a number of crucial steps of B-cell terminal differentiation needed for efficient antibody responses. AICDA has a role in the epigenetic regulation of gene expression by participating in DNA demethylation. AICDA is strongly expressed in the lymph nodes and tonsils.
AICDA Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (1-198) and having a molecular mass of 26.1kDa.AICDA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | AID: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Strongly expressed in lymph nodes and tonsils. Belongs to the cytidine and deoxycytidylate deaminase family. |
| UniProt Protein Details: | Protein type:EC 3.5.4.38; Hydrolase Chromosomal Location of Human Ortholog: 12p13 Cellular Component: cytoplasm; exosome (RNase complex); nucleus Molecular Function:protein binding; zinc ion binding; ubiquitin protein ligase binding; cytidine deaminase activity Biological Process: somatic diversification of immunoglobulins; B cell differentiation; somatic hypermutation of immunoglobulin genes; isotype switching; cytidine deamination; mRNA processing Disease: Immunodeficiency With Hyper-igm, Type 2 |
| NCBI Summary: | This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009] |
| UniProt Code: | Q9GZX7 |
| NCBI GenInfo Identifier: | 23813666 |
| NCBI Gene ID: | 57379 |
| NCBI Accession: | Q9GZX7.1 |
| UniProt Related Accession: | Q9GZX7 |
| Molecular Weight: | |
| NCBI Full Name: | Single-stranded DNA cytosine deaminase |
| NCBI Synonym Full Names: | activation induced cytidine deaminase |
| NCBI Official Symbol: | AICDA�� |
| NCBI Official Synonym Symbols: | AID; ARP2; CDA2; HIGM2; HEL-S-284�� |
| NCBI Protein Information: | single-stranded DNA cytosine deaminase |
| UniProt Protein Name: | Single-stranded DNA cytosine deaminase |
| UniProt Synonym Protein Names: | Activation-induced cytidine deaminase; Cytidine aminohydrolase |
| Protein Family: | AIDA-I autotransporter |
| UniProt Gene Name: | AICDA�� |
| UniProt Entry Name: | AICDA_HUMAN |
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