Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) CLIA Kit
The Human Acidic Fibroblast Growth Factor 1 (FGF1) CLIA Kit is a reliable and accurate tool for measuring levels of FGF1 in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing precise and consistent results for various research applications.FGF1, also known as acidic fibroblast growth factor, is a key player in cell growth, differentiation, and wound healing processes. Its dysregulation has been implicated in numerous diseases, including cancer, cardiovascular diseases, and developmental disorders.
Therefore, measuring FGF1 levels can offer valuable insights into disease mechanisms and potential therapeutic targets.The Human FGF1 CLIA Kit from AssayGenie is a valuable tool for researchers studying FGF1's role in disease pathogenesis and for those working on developing novel treatments targeting the FGF1 pathway. Trust in the accuracy and reliability of this kit to enhance your research and advance scientific knowledge in the field of growth factor signaling.
Product Name:
Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) CLIA Kit
SKU:
HUES00069
Target:
Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AFGF/FGF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AFGF/FGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFGF/FGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AFGF/FGF1. You can calculate the concentration of Human AFGF/FGF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
90-104
91-104
Average (%)
90
95
97
1:4
Range (%)
99-115
93-103
89-100
Average (%)
106
98
94
1:8
Range (%)
88-102
92-105
101-118
Average (%)
94
97
108
1:16
Range (%)
93-106
91-105
87-100
Average (%)
99
97
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
103
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.84
82.3
327.26
40.45
89.02
353.66
Standard deviation
4.36
6.32
28.01
4.91
8.46
37.84
C V (%)
10.18
7.68
8.56
12.14
9.5
10.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human AFGF/FGF1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human AFGF/FGF1 in samples. No significant cross-reactivity or interference between Human AFGF/FGF1 and analogues was observed.
