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Human Adrenergic Receptor Beta 1 (ADRb1) ELISA Kit

SKU:
HUDL00093
Product Type:
ELISA Kit
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ADR-B1,ADRB1R,B1AR,BETA1AR,RHR,Beta 1 Adrenoreceptor,Beta-1 adrenoceptor
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Adrenergic Receptor Beta 1 (ADRb1) ELISA Kit

The Human Adrenergic Receptor Beta-1 (ADRB1) ELISA Kit is specifically designed for accurate detection of ADRB1 levels in human serum, plasma, and cell culture supernatants. This advanced kit provides high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.ADRB1 is a key receptor involved in the sympathetic nervous system and plays a crucial role in regulating heart function and blood pressure. Dysregulation of ADRB1 has been implicated in various cardiovascular conditions, making it a valuable biomarker for studying these diseases and potentially developing new therapeutic strategies.

With the Human Adrenergic Receptor Beta-1 ELISA Kit, researchers can easily quantify ADRB1 levels in biological samples, allowing for precise measurements and deeper insights into the role of this receptor in health and disease. This kit is a valuable tool for advancing cardiovascular research and personalized medicine initiatives.

Product Name:Human Adrenergic Receptor Beta 1 (ADRb1) ELISA Kit
Product Code:HUDL00093
Size:96 Assays
Target:ADRb1
Synonyms:ADR-B1, ADRB1R, B1AR, BETA1AR, RHR, Beta 1 Adrenoreceptor, Beta-1 adrenoceptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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