Human ADRA1A (Adrenergic Receptor alpha-1A ) CLIA Kit
The Human ADRA1A (Adrenergic Receptor Alpha 1A) CLIA Kit is designed for the accurate and efficient detection of ADRA1A levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.The ADRA1A protein is a key player in the adrenergic signaling pathway, regulating various physiological processes such as smooth muscle contraction, neurotransmitter release, and cardiac function.
Dysregulation of ADRA1A has been implicated in several disorders, including hypertension, prostate cancer, and heart failure, making it a valuable biomarker for studying these conditions and exploring potential therapeutic strategies.Overall, the Human ADRA1A CLIA Kit provides researchers with a powerful tool for investigating the role of ADRA1A in health and disease, offering valuable insights into its function and potential as a therapeutic target.
Product Name:
Human ADRA1A (Adrenergic Receptor alpha-1A ) CLIA Kit
SKU:
HUES00205
Target:
Human ADRA1A (Adrenergic Receptor alpha-1A )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADRA1A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADRA1A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADRA1A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ADRA1A. You can calculate the concentration of Human ADRA1A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-113
85-95
86-100
Average (%)
107
90
93
1:4
Range (%)
97-112
96-113
90-106
Average (%)
104
103
97
1:8
Range (%)
89-100
96-110
88-100
Average (%)
94
101
95
1:16
Range (%)
102-117
99-112
89-106
Average (%)
110
106
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
105
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
98-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
185.44
371.17
1994.5
194.54
337.46
2101.72
Standard deviation
16.58
33.52
220.39
19.42
30.24
217.74
C V (%)
8.94
9.03
11.05
9.98
8.96
10.36
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ADRA1A concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human ADRA1A in samples. No significant cross-reactivity or interference between Human ADRA1A and analogues was observed.
