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Human ADP Ribosyltransferase 1 (ART1) ELISA Kit

SKU:
HUDL00253
Product Type:
ELISA Kit
Sensitivity:
0.234ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
CD296,ART2,ARTC1,Mono(ADP-ribosyl)transferase 1,ADP-ribosyltransferase C2 and C3 toxin-like 1,GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

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Human ADP Ribosyltransferase 1 (ART1) ELISA Kit

The Human ADP Ribosyltransferase 1 (ART1) ELISA Kit is specifically designed for the precise measurement of ART1 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an indispensable tool for a variety of research applications.ART1 is an important enzyme involved in the ADP-ribosylation process, a post-translational modification that plays a key role in various cellular functions.

Dysregulation of ART1 has been implicated in diseases such as cancer, inflammatory disorders, and autoimmune conditions, highlighting its significance as a potential therapeutic target and biomarker for disease diagnosis and prognosis.By utilizing the Human ADP Ribosyltransferase 1 (ART1) ELISA Kit, researchers can gain valuable insights into the role of ART1 in health and disease, advancing our understanding of cellular mechanisms and paving the way for innovative treatment strategies.

Product Name:Human ADP Ribosyltransferase 1 (ART1) ELISA Kit
Product Code:HUDL00253
Size:96 Assays
Target:ART1
Synonyms:CD296, ART2, ARTC1, Mono(ADP-ribosyl)transferase 1, ADP-ribosyltransferase C2 and C3 toxin-like 1, GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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