The Human Adipor2 (Adiponectin Receptor 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Adipor2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides researchers with reliable and reproducible results, making it suitable for a wide range of research applications.Adipor2 is a key receptor involved in the regulation of adiponectin, a hormone that plays a crucial role in metabolic processes and insulin sensitivity. Dysregulation of the adiponectin pathway has been linked to various metabolic disorders such as obesity, diabetes, and cardiovascular diseases, highlighting the importance of studying Adipor2 levels in these conditions.
By utilizing the Human Adipor2 CLIA Kit, researchers can gain valuable insights into the role of Adipor2 in metabolic disorders and potentially identify new therapeutic targets for the treatment of these conditions. With its high sensitivity and specificity, this kit offers an efficient and accurate solution for studying Adipor2 levels in human samples.
Product Name:
Human ADIPOR2 (Adiponectin Receptor 2) CLIA Kit
SKU:
HUES00203
Target:
Human ADIPOR2 (Adiponectin Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ADIPOR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADIPOR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADIPOR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ADIPOR2. You can calculate the concentration of Human ADIPOR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
93-109
102-116
Average (%)
105
101
108
1:4
Range (%)
95-109
99-111
99-118
Average (%)
101
104
107
1:8
Range (%)
87-99
83-97
95-110
Average (%)
94
90
101
1:16
Range (%)
92-106
91-105
88-101
Average (%)
97
97
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
101
EDTA plasma (n=5)
96-112
104
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.15
4.89
0.54
1.22
4.86
Standard deviation
0.04
0.1
0.52
0.05
0.1
0.4
C V (%)
7.27
8.7
10.63
9.26
8.2
8.23
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ADIPOR2 concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human ADIPOR2 in samples. No significant cross-reactivity or interference between Human ADIPOR2 and analogues was observed.
