Human ADI1 Recombinant Protein (RPPB1358)
- SKU:
- RPPB1358
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9BV57
- Research Area:
- Enzymes
Description
| Product Name: | Human ADI1 Recombinant Protein |
| Product Code: | RPPB1358 |
| Size: | 20µg |
| Species: | Human |
| Target: | ADI1 |
| Synonyms: | APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL, 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase, Acireductone dioxygenase, Fe-ARD, Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1, Submergence-induced protein-like factor. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The ADI1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSMVL AWYMDDAPGD PRQPHRPDPG RPVGLEQLRR LGVLYWKLDA DKYENDPELE KIRRERNYSW MDIITICKDK LPNYEEKIKM FYEEHLHLDD EIRYILDGSG YFDVRDKEDQ WIRIFMEKGD MVTLPAGIYH RFTVDEKNYT KAMRLFVGEP VWTAYNRPAD HFEARGQYVK FLAQTA |
Acireductone dioxygenase 1 (ADI1) is a part of the acireductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. ADI1 regulates mRNA processing in the nucleus, and carries out different functions depending on its localization. Related pseudogenes have been defined on chromosomes 8 and 20. ADI1 down-regulates cell migration arbitrated by MMP14.
ADI1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 216 amino acids (1-179 a.a.) and having a molecular mass of 25.6kDa. ADI1 is fused to a 37 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ADI1: Catalyzes the formation of formate and 2-keto-4- methylthiobutyrate (KMTB) from 1,2-dihydroxy-3-keto-5- methylthiopentene (DHK-MTPene). Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line. Belongs to the acireductone dioxygenase (ARD) family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - cysteine and methionine; EC 1.13.11.54; Oxidoreductase Chromosomal Location of Human Ortholog: 2p25.3 Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; plasma membrane; nucleus; cytosol Molecular Function:acireductone dioxygenase [iron(II)-requiring] activity; protein binding; iron ion binding; oxidoreductase activity Biological Process: methionine salvage; sulfur amino acid metabolic process; polyamine metabolic process |
| UniProt Code: | Q9BV57 |
| NCBI GenInfo Identifier: | 74733289 |
| NCBI Gene ID: | 55256 |
| NCBI Accession: | Q9BV57.1 |
| Molecular Weight: | 21kDa |
| NCBI Full Name: | 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase |
| UniProt Protein Name: | 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase |
| UniProt Synonym Protein Names: | Acireductone dioxygenase (Fe(2+)-requiring); Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein 1 |
| Protein Family: | 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase |
| UniProt Gene Name: | ADI1�� |
| UniProt Entry Name: | MTND_HUMAN |
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