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Human ADCYAP1/PACAP (Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit

SKU:
AEES00026
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Human
Sensitivity:
4.69 pg/mL
Range:
7.81-500 pg/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Human ADCYAP1/PACAP (Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit

The ADCYAP1/PACAP ELISA Kit is designed for the quantitative detection of Adenylate Cyclase Activating Polypeptide 1 (ADCYAP1/PACAP) levels in various human biological samples.

ADCYAP1/PACAP is a neuropeptide that performs diverse biological activities, including the regulation of adrenal gland function, cardiovascular activity, and neurotransmission. It is implicated in numerous physiological processes and disease states, making accurate measurement of ADCYAP1/PACAP essential for understanding its role in health and disease. Assay Genie's ADCYAP1/PACAP ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under rigorous quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's ADCYAP1/PACAP ELISA Kit for accurate and reliable quantification of this pivotal biomarker in your studies.

Product Name:Human ADCYAP1/PACAP (Adenylate Cyclase Activating Polypeptide 1, Pituitary) ELISA Kit
Product Code:AEES00026
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Human
Detection Range:7.81-500 pg/mL
Sensitivity:4.69 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Human ADCYAP1/PACAP in samples. No significant cross-reactivity or interference between Human ADCYAP1/PACAP and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Human ADCYAP1/PACAP concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADCYAP1/PACAP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ADCYAP1/PACAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADCYAP1/PACAP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ADCYAP1/PACAP. You can calculate the concentration of Human ADCYAP1/PACAP in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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