Human ACTN3 (Actinin Alpha 3) CLIA Kit (HUES00192)
- SKU:
- HUES00192
- Product Type:
- ELISA Kit
- ELISA Type:
- CLIA Kit
- Size:
- 96 Assays
- Sensitivity:
- 46.88pg/mL
- Range:
- 78.13-5000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, MGC117002, MGC117005
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Cell Biology
Description
Product Name: | Human ACTN3 (Actinin Alpha 3) CLIA Kit |
SKU: | HUES00192 |
Target: | Human ACTN3 (Actinin Alpha 3) |
Size: | 96T |
Assay type: | Sandwich |
Assay time: | 4.5h |
Sensitivity: | 46.88 pg/mL |
Detection range: | 78.13-5000 pg/mL |
Kit component: |
|
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACTN3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTN3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTN3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACTN3. You can calculate the concentration of Human ACTN3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity: |
| ||||||||||||||||||||||||||||||||||||||||||
Recovery: |
| ||||||||||||||||||||||||||||||||||||||||||
Precision: |
|
Sample type &Sample volume: | Tissue homogenates; 100μL |
Reproducibility: | Both intra-CV and inter-CV are < 15%. |
Application: | This CLIA kit applies to the in vitro quantitative determination of Human ACTN3 concentrations in Tissue homogenates. |
Specificity: | This kit recognizes Human ACTN3 in samples. No significant cross-reactivity or interference between Human ACTN3 and analogues was observed. |