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Human Activating Transcription Factor 4 (ATF4) ELISA Kit

SKU:
HUDL00272
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P18848
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TAXREB67,TXREB,CREB-2,DNA-binding protein TAXREB67,Cyclic AMP-responsive element-binding protein 2,Tax-responsive enhancer element-binding protein 67
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Human Activating Transcription Factor 4 (ATF4) ELISA Kit

The Human Activating Transcription Factor 4 (ATF4) ELISA Kit is a powerful tool for the precise measurement of ATF4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results that are essential for various research applications.ATF4 is a key transcription factor that regulates the expression of genes involved in stress responses, metabolism, and cell survival.

Its dysregulation has been linked to various diseases, including cancer, diabetes, and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its dependable performance and ease of use, the Human ATF4 ELISA Kit from Assay Genie is an indispensable tool for researchers investigating the role of ATF4 in health and disease.

Product Name:Human Activating Transcription Factor 4 (ATF4) ELISA Kit
Product Code:HUDL00272
Size:96 Assays
Target:ATF4
Synonyms:TAXREB67, TXREB, CREB-2, DNA-binding protein TAXREB67, Cyclic AMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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