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Human Actin Beta (ACTb) ELISA Kit

SKU:
HUDL00046
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P60709
Sensitivity:
0.63ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
ACT-B,PS1TP5BP1,Actin, cytoplasmic 1,Actin, cytoplasmic 1, N-terminally processed
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Actin Beta (ACTb) ELISA Kit

The Human β-Actin (ACTB) ELISA Kit is specially designed for the precise measurement of β-actin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.β-Actin is a fundamental component of the cytoskeleton and is involved in cell structure, movement, and signaling processes. It plays a critical role in cellular functions and is often used as a loading control in Western blotting and other experimental techniques.

With its reliable performance and broad applicability, the Human β-Actin (ACTB) ELISA Kit is an indispensable tool for studying cellular processes, biomarker discovery, and drug development in various fields such as cancer research, cell biology, and immunology. Order yours today to enhance your research outcomes.

Product Name:Human Actin Beta (ACTb) ELISA Kit
Product Code:HUDL00046
Size:96 Assays
Target:ACTb
Synonyms:ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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