Human ACTH (Adrenocorticotropic Hormone) ELISA Kit
The Human ACTH (Adrenocorticotropic Hormone) ELISA Kit is a high-quality assay designed for the precise detection of ACTH levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.ACTH is a key hormone involved in the regulation of the body's stress response and plays a crucial role in the synthesis and release of cortisol from the adrenal glands.
Dysregulation of ACTH levels can contribute to conditions such as Cushing's syndrome and other adrenal disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.With its advanced technology and ease of use, the Human ACTH ELISA Kit from Assay Genie is an essential tool for researchers investigating the intricate mechanisms of hormone regulation and looking to advance our understanding of various endocrine disorders.
Product Name:
Human ACTH (Adrenocorticotropic Hormone) ELISA Kit
SKU:
HUES01414
Target:
Human ACTH (Adrenocorticotropic Hormone)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ACTH. During the reaction, ACTH in the sample or standard competes with a fixed amount of ACTH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ACTH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
92-106
97-110
Average (%)
94
97
104
1:4
Range (%)
89-103
94-107
96-111
Average (%)
95
100
102
1:8
Range (%)
85-97
88-101
92-110
Average (%)
90
93
100
1:16
Range (%)
90-100
90-103
95-107
Average (%)
95
98
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
85-100
91
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.7
130.9
461.6
45.1
124.7
448.6
Standard deviation
3.2
6.8
14.3
2.3
6.4
19.7
C V (%)
7
5.19
3.1
5.1
5.13
4.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of ACTH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes ACTH in samples. No significant cross-reactivity or interference between ACTH and analogues was observed.
