Human ACP3/PAP (Prostatic Acid Phosphatase 3) CLIA Kit
The Human ACP3 (Prostatic Acid Phosphatase 3) CLIA Kit is specifically designed for the accurate detection of ACP3 levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring precise and reliable results for various research applications.ACP3, also known as prostatic acid phosphatase 3, is an important enzyme involved in various biological processes, including prostate health and bone metabolism. It serves as a biomarker for prostate cancer and other related conditions, making it a valuable tool for understanding disease progression and developing targeted therapies.
With its advanced technology and user-friendly protocol, the Human ACP3 CLIA Kit from Assay Genie is an essential tool for researchers and clinicians studying ACP3 and its implications in human health. Order yours today for accurate and efficient detection of ACP3 levels in biological samples.
Product Name:
Human ACP3/PAP (Prostatic Acid Phosphatase 3) CLIA Kit
SKU:
HUES01021
Target:
Human ACP3/PAP (Prostatic Acid Phosphatase 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ACP3/PAP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACP3/PAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACP3/PAP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ACP3/PAP. You can calculate the concentration of Human ACP3/PAP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
87-100
94-109
Average (%)
98
93
101
1:4
Range (%)
96-110
86-98
86-99
Average (%)
103
92
93
1:8
Range (%)
95-105
93-109
100-113
Average (%)
100
101
106
1:16
Range (%)
84-97
97-111
87-100
Average (%)
90
103
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
102
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.32
3.48
0.51
1.34
3.39
Standard deviation
0.06
0.13
0.32
0.06
0.14
0.22
C V (%)
12.77
9.85
9.2
11.76
10.45
6.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ACP3/PAP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACP3/PAP in samples. No significant cross-reactivity or interference between Human ACP3/PAP and analogues was observed.
