Human AChE (Acetylcholinesterase) ELISA Kit (AEES00067)

Product Name:	Human AChE (Acetylcholinesterase) ELISA Kit
SKU:	AEES00067
Target:	Human AChE (Acetylcholinesterase)
Size:	96T
Assay type:	Sandwich-ELISA
Assay time:	3.5h
Sensitivity:	9.38 IU/mL
Detection range:	15.63-1000 IU/mL

Kit component:

Component	Quantity (24 Assays)	Quantity (96 Assays)	Storage
Micro ELISA Plate (Dismountable)	8 wells x 3 strips	8 wells x 12 strips	-20°C, 12 months
Reference Standard	1 vial	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 60 µL	1 vial, 120 µL
Concentrated HRP Conjugate (100×)	1 vial, 60 µL	1 vial, 120 µL	-20°C (shading light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	1 vial, 20 mL	4°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	1 vial, 10 mL	4°C (shading light)
Stop Solution	1 vial, 10 mL	1 vial, 10 mL	4°C
Plate Sealer	5 pieces	5 pieces
Product Description	1 copy	1 copy

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AChE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AChE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AChE. You can calculate the concentration of Human AChE in the samples by comparing the OD of the samples to the standard curve.

Linearity:

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	93-100	86-100	91-104
1:2	Average (%)	97	94	96
1:4	Range (%)	98-107	92-104	88-100
1:4	Average (%)	101	97	92
1:8	Range (%)	94-107	100-106	96-108
1:8	Average (%)	100	103	101
1:16	Range (%)	93-105	88-98	85-97
1:16	Average (%)	101	91	92

Recovery:

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	89-101	97
EDTA plasma (n=5)	97-107	100
Cell culture media (n=5)	85-98	92

Precision:

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20.0	20.0	20.0	20.0	20.0
Mean (ng/mL)	42.96	103.71	476.6	47.71	120.4	458.41
Standard deviation	2.2	5.05	23.83	2.84	5.78	22.55
C V (%)	5.12	4.87	5.0	5.95	4.8	4.92

Sample type &Sample volume:	serum, plasma and other biological fluids; 100μL
Reproducibility:	Both intra-CV and inter-CV are < 10%.
Application:	This ELISA kit applies to the in vitro quantitative determination of Human AChE concentrations in serum, plasma and other biological fluids.
Specificity:	This kit recognizes Human AChE in samples. No significant cross-reactivity or interference between Human AChE and analogues was observed.