Human ACE (Angiotensin Ⅰ Converting Enzyme) ELISA Kit
The Assay Genie Human ACE (Angiotensin I Converting Enzyme) ELISA Kit is expertly designed for the precise quantitative analysis of ACE levels in a variety of biological samples. ACE, known as Angiotensin I Converting Enzyme, is a vital enzyme in the renin-angiotensin system, playing a crucial role in regulating blood pressure and maintaining cardiovascular homeostasis. By accurately measuring ACE levels, researchers can gain valuable insights into its involvement in cardiovascular health, hypertension, and renal function, influencing the development of targeted therapeutic interventions in these critical areas.
Our ACE ELISA Kit ensures exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for your research endeavors. Crafted under strict quality control measures, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for conducting in-depth investigations into ACE biology. Trust Assay Genie's ACE ELISA Kit for reliable and precise quantification, empowering your scientific exploration into this essential enzyme.
Product Name:
Human ACE (Angiotensin Ⅰ Converting Enzyme) ELISA Kit
SKU:
AEES00178
Target:
Human ACE (Angiotensin Ⅰ Converting Enzyme)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ACE. You can calculate the concentration of Human ACE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
94-107
84-99
Average (%)
102
99
91
1:4
Range (%)
97-109
90-106
83-93
Average (%)
103
98
88
1:8
Range (%)
99-114
94-107
84-94
Average (%)
105
100
89
1:16
Range (%)
91-104
91-104
81-93
Average (%)
97
99
86
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.33
9.01
1.13
2.11
9.64
Standard deviation
0.06
0.13
0.44
0.08
0.1
0.32
C V (%)
5.22
5.44
4.92
6.86
4.72
3.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ACE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ACE in samples. No significant cross-reactivity or interference between Human ACE and analogues was observed.
