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Human 5-Hydroxytryptamine Receptor 1A (HTR1A) ELISA Kit

SKU:
HUDL01403
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08908
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
5HTR1A,5HTR1-A,5-HTR1-A,ADRB2RL1,ADRBRL1,Serotonin Receptor 1A
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human 5-Hydroxytryptamine Receptor 1A (HTR1A) ELISA Kit

The Human 5-Hydroxytryptamine Receptor 1A (HTR1A) ELISA Kit is specifically designed for the accurate measurement of 5-HT receptor levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.The 5-HT receptor 1A is a key player in the serotonergic system, influencing a wide range of physiological processes such as mood regulation, cognition, and behavior.

Dysregulation of this receptor has been linked to various psychiatric disorders, including depression, anxiety, and schizophrenia, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its reliable performance and broad applicability, the Human 5-Hydroxytryptamine Receptor 1A (HTR1A) ELISA Kit is an essential tool for researchers investigating the role of serotonin receptors in health and disease.

Product Name:Human 5-Hydroxytryptamine Receptor 1A (HTR1A) ELISA Kit
Product Code:HUDL01403
Size:96 Assays
Target:HTR1A
Synonyms:5HTR1A, 5HTR1-A, 5-HTR1-A, ADRB2RL1, ADRBRL1, Serotonin Receptor 1A
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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