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Human Arachidonate-15-Lipoxygenase (ALOX15) ELISA Kit

SKU:
HUDL00145
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P16050
Sensitivity:
0.57ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
LOX15,15-LOX2,15-LOX1,15-LOX,12-LOX,ALOX15,15-LO,15-LPG,LOG15,Lipoxygenase 15,Arachidonate 12-lipoxygenase, leukocyte-type,Arachidonate omega-6 lipoxygenase
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Arachidonate-15-Lipoxygenase (ALOX15) ELISA Kit

The Human Arachidonate 15-Lipoxygenase (ALOX15) ELISA Kit is specifically designed for the precise quantification of ALOX15 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for a wide range of research purposes.ALOX15 is an important enzyme involved in the metabolism of arachidonic acid, playing a key role in inflammation and immune response regulation.

Dysregulation of ALOX15 has been implicated in various diseases including asthma, atherosclerosis, and cancer, making it a critical biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Human Arachidonate 15-Lipoxygenase (ALOX15) ELISA Kit provides researchers with a reliable and efficient method for measuring ALOX15 levels, facilitating in-depth investigations into its physiological functions and pathological implications.

Product Name:Human Arachidonate-15-Lipoxygenase (ALOX15) ELISA Kit
Product Code:HUDL00145
Size:96 Assays
Target:ALOX15
Synonyms:LOX15, 15-LOX2, 15-LOX1, 15-LOX, 12-LOX, ALOX15, 15-LO, 15-LPG, LOG15, Lipoxygenase 15, Arachidonate 12-lipoxygenase, leukocyte-type, Arachidonate omega-6 lipoxygenase
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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