The HTT Polyclonal Antibody (CAB16872) is a powerful tool for researchers studying the huntingtin protein, which is associated with Huntington's disease. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the HTT protein, this antibody enables precise detection and analysis in various cell types, making it invaluable for studies in neurobiology and neurodegenerative diseases.Huntingtin is a large protein involved in intracellular transport and signaling, but an abnormal form of huntingtin is implicated in the development of Huntington's disease, a devastating neurodegenerative disorder.
By studying the function and regulation of HTT, researchers aim to deepen our understanding of the disease mechanisms and potentially identify new therapeutic targets for treating Huntington's and other related conditions. The HTT Polyclonal Antibody offers a reliable tool for advancing research in this crucial field.
Product Name:
Huntingtin Rabbit Polyclonal Antibody
SKU:
CAB16872
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 435-635 of human Huntingtin (NP_002102.4).
Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range of trinucleotide repeats (9-35) has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression.
Purification Method:
Affinity purification
Gene ID:
3064
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Huntingtin Rabbit pAb (CAB16872) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
