The HTR2C Polyclonal Antibody is a powerful tool for researchers studying the serotonin receptor 2C (HTR2C) protein. This antibody, generated in rabbits, is highly specific to HTR2C and is validated for use in Western blot applications. By targeting the HTR2C protein, this antibody allows for the detection and analysis of HTR2C in various cell types, making it an ideal choice for studies in neuroscience, psychiatry, and drug development.The HTR2C protein is a key player in the regulation of serotonin signaling in the brain, influencing a wide range of functions including mood, appetite, and sleep.
Dysregulation of HTR2C has been implicated in various psychiatric disorders such as depression, anxiety, and schizophrenia, making it a promising target for therapeutic interventions. By studying the role of HTR2C using this antibody, researchers can gain valuable insights into the underlying mechanisms of these disorders and potentially identify novel treatment strategies.
Product Name:
HTR2C Polyclonal Antibody
SKU:
CAB11831
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide of human HTR2C
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
HTR1C; 5-HT1C; 5-HT2C; 5HTR2C; 5-HTR2C; HTR2C
Positive Sample:
SH-SY5Y,A-431,U-87MG,Jurkat,B cells
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Multi-pass membrane protein.
Calculated MW:
52kDa
Observed MW:
75kDa
This gene encodes a seven-transmembrane G-protein-coupled receptor. The encoded protein responds to signaling through the neurotransmitter serotonin. The mRNA of this gene is subject to multiple RNA editing events, where adenosine residues encoded by the genome are converted to inosines. RNA editing is predicted to alter the structure of the second intracellular loop, thereby generating alternate protein forms with decreased ability to interact with G proteins. Abnormalities in RNA editing of this gene have been detected in victims of suicide that suffer from depression. In addition, naturally-occuring variation in the promoter and 5' non-coding and coding regions of this gene may show statistically-significant association with mental illness and behavioral disorders. Alternative splicing results in multiple different transcript variants.
Purification Method:
Affinity purification
Gene ID:
3358
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HTR2C antibody (CAB11831) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
