The HSPB8/HSP22 Rabbit Monoclonal Antibody is a powerful tool for researchers studying heat shock proteins and their role in cellular stress response. This antibody is raised in rabbits and is highly specific for HSPB8, also known as HSP22, a small heat shock protein involved in protein folding and protection against cell stress. Validation studies have shown that this antibody is suitable for use in a variety of applications, including Western blot, immunohistochemistry, and immunofluorescence. Its high reactivity with human samples makes it an ideal choice for researchers investigating the function of HSPB8 in human health and disease.
Heat shock proteins like HSPB8 play a crucial role in maintaining cellular homeostasis and protecting cells from damage caused by environmental stressors. By using this monoclonal antibody to study HSPB8, researchers can gain valuable insights into the mechanisms of cellular stress response and potentially identify new therapeutic targets for conditions related to protein misfolding and cellular damage.
Product Name:
HSPB8/HSP22 Rabbit Monoclonal Antibody
SKU:
CAB13518
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HSPB8/HSP22 (Q9UJY1).
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
Purification Method:
Affinity purification
Gene ID:
26353
Clone Number:
ARC0715
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HSPB8/HSP22 Rabbit mAb (CAB13518) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
