Anti-HSPB2 Antibody (CAB2350)
- SKU:
- CAB2350
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
Anti-HSPB2 Antibody (CAB2350)
The HSPB2 Polyclonal Antibody (CAB2350) is a valuable tool for research focused on the heat shock protein beta-2 (HSPB2), a member of the small heat shock protein family implicated in cellular stress response and muscle function. This antibody, generated in rabbits, exhibits strong reactivity towards human samples and has been validated for use in Western blot applications. By specifically binding to the HSPB2 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an ideal choice for investigations in cell biology, molecular biology, and physiology.HSPB2, also known as heat shock protein 27 (HSP27), is involved in protecting cells from stress-induced damage and maintaining protein homeostasis. Its role in promoting cell survival and regulating muscle function underscores its importance in various physiological processes, including muscle development and function, as well as cellular response to stressors like heat shock and oxidative stress.
Research into HSPB2 can provide valuable insights into mechanisms of cellular stress response and potential therapeutic interventions for conditions related to protein misfolding and cellular damage.Overall, the HSPB2 Polyclonal Antibody (CAB2350) is a reliable tool for studying the functions and regulation of the HSPB2 protein in different biological contexts, offering valuable information for advancing our understanding of cellular stress response mechanisms and their implications for human health.
| Antibody Name: | Anti-HSPB2 Antibody |
| Antibody SKU: | CAB2350 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human HSPB2 (NP_001532.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, HeLa, Mouse skeletal muscle |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human HSPB2 (NP_001532.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGR SVPH AHPA TAEY EFAN PSRL GEQR FGEG LLPE EILT PTLY HGYY VRPR AAPA GEGS RAGA SELR LSEG KFQA FLDV SHFT PDEV TVRT VDNL LEVS ARHP QRLD RHGF VSRE FCRT YVLP ADVD PWRV RAAL SHDG ILNL EAPR GGRH LDTE VNEV YISL LPAP PDPE EEEE AAIV EP |
| Gene ID: | 3316 |
| Uniprot: | Q16082 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 20kDa |
| Observed MW: | 20kDa |
| Synonyms: | HSPB2, HSP27, Hs.78846, LOH11CR1K, MKBP |
| Background: | The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The protein is expressed preferentially in the heart and skeletal muscle. This protein regulates Myotonic Dystrophy Protein Kinase, which plays an important role in maintenance of muscle structure and function. |
| UniProt Protein Function: | HSPB2: May regulate the kinase DMPK. Belongs to the small heat shock protein (HSP20) family. |
| UniProt Protein Details: | Protein type:Heat shock protein; Chaperone Chromosomal Location of Human Ortholog: 11q22-q23 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:enzyme activator activity; protein binding Biological Process: positive regulation of catalytic activity; response to heat; response to unfolded protein; somatic muscle development |
| NCBI Summary: | The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The protein is expressed preferentially in the heart and skeletal muscle. This protein regulates Myotonic Dystrophy Protein Kinase, which plays an important role in maintenance of muscle structure and function. [provided by RefSeq, Dec 2012] |
| UniProt Code: | Q16082 |
| NCBI GenInfo Identifier: | 6016268 |
| NCBI Gene ID: | 3316 |
| NCBI Accession: | Q16082.2 |
| UniProt Secondary Accession: | Q16082,Q6I9U7, |
| UniProt Related Accession: | Q16082 |
| Molecular Weight: | 20,233 Da |
| NCBI Full Name: | Heat shock protein beta-2 |
| NCBI Synonym Full Names: | heat shock protein family B (small) member 2 |
| NCBI Official Symbol: | HSPB2Â Â |
| NCBI Official Synonym Symbols: | MKBP; HSP27; Hs.78846; LOH11CR1KÂ Â |
| NCBI Protein Information: | heat shock protein beta-2 |
| UniProt Protein Name: | Heat shock protein beta-2 |
| UniProt Synonym Protein Names: | DMPK-binding protein; MKBP |
| Protein Family: | Heat shock protein |
| UniProt Gene Name: | HSPB2Â Â |
| UniProt Entry Name: | HSPB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HSPB2 antibody (CAB2350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded mouse heart using HSPB2 antibody (CAB2350) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using HSPB2 antibody (CAB2350). Blue: DAPI for nuclear staining. |
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