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Anti-BiP/GRP78 Antibody (CAB11568)

SKU:
CAB11568
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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Anti-BiP/GRP78 Antibody (CAB11568)

The HSPA5 Polyclonal Antibody (CAB11568) is a valuable tool for researchers investigating HSPA5, also known as heat shock 70 kDa protein 5. This cell surface molecule is involved in protein folding and trafficking, making it essential for cellular homeostasis and stress response. The antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the HSPA5 protein, this antibody enables researchers to detect and analyze HSPA5 expression in various cell types, making it ideal for studies in molecular biology and cell biology.

HSPA5 plays a crucial role in maintaining protein quality control and protecting cells from stress-induced damage. Dysregulation of HSPA5 has been linked to various diseases, including neurodegenerative disorders and cancer. By studying the function of HSPA5, researchers can gain insights into disease mechanisms and potentially identify therapeutic targets for interventions. The HSPA5 Polyclonal Antibody is a valuable tool for advancing our understanding of cellular stress response and its implications for human health.

Antibody Name:Anti-BiP/GRP78 Antibody
Antibody SKU:CAB11568
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human BiP/GRP78
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, Mouse testis
Immunogen:Recombinant protein of human BiP/GRP78
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3309
Uniprot:P11021
Cellular Location:Cytoplasm, Endoplasmic reticulum lumen, Melanosome
Calculated MW:72kDa
Observed MW:72kDa
Synonyms:HSPA5, BIP, GRP78, HEL-S-89n, MIF2
Background:The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.
UniProt Protein Function:GRP78: a member of the HSP family of molecular chaperones required for endoplasmic reticulum integrity and stress-induced autophagy. Plays a central role in regulating the unfolded protein response (UPR), and is an obligatory component of autophagy in mammalian cells. May play an important role in cellular adaptation and oncogenic survival. One of the client proteins of GRP78 is protein double-stranded RNA-activated protein-like endoplasmic reticulum kinase (PERK). Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
UniProt Protein Details:

Protein type:Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: signalosome; endoplasmic reticulum membrane; focal adhesion; smooth endoplasmic reticulum; cell surface; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment; membrane; melanosome; plasma membrane; integral to endoplasmic reticulum membrane; midbody; nucleus

Molecular Function:protein domain specific binding; protein binding; enzyme binding; chaperone binding; ubiquitin protein ligase binding; unfolded protein binding; ATPase activity; ribosome binding; calcium ion binding; misfolded protein binding; glycoprotein binding; ATP binding

Biological Process: platelet activation; ER-associated protein catabolic process; cerebellum structural organization; unfolded protein response; cerebellar Purkinje cell layer development; cellular response to glucose starvation; platelet degranulation; substantia nigra development; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; positive regulation of protein ubiquitination; blood coagulation; negative regulation of transforming growth factor beta receptor signaling pathway; ER overload response; positive regulation of cell migration; negative regulation of apoptosis

NCBI Summary:The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
UniProt Code:P11021
NCBI GenInfo Identifier:14916999
NCBI Gene ID:3309
NCBI Accession:P11021.2
UniProt Secondary Accession:P11021,Q2EF78, Q9NPF1, Q9UK02, B0QZ61,
UniProt Related Accession:P11021
Molecular Weight:
NCBI Full Name:78 kDa glucose-regulated protein
NCBI Synonym Full Names:heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
NCBI Official Symbol:HSPA5  
NCBI Official Synonym Symbols:BIP; MIF2; GRP78; HEL-S-89n  
NCBI Protein Information:78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; epididymis secretory sperm binding protein Li 89n; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
UniProt Protein Name:78 kDa glucose-regulated protein
UniProt Synonym Protein Names:Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
UniProt Gene Name:HSPA5  
UniProt Entry Name:GRP78_HUMAN
Anti-BiP/GRP78 Antibody (CAB11568)Western blot analysis of extracts of various cell lines, using BiP/GRP78 antibody (CAB11568) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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