The HSPA5 Polyclonal Antibody (CAB11366) is specifically designed for research involving HSPA5, also known as heat shock 70 kDa protein 5, a key protein involved in cellular stress response and protein folding. The antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. HSPA5 is a chaperone protein which plays a critical role in assisting the correct folding of newly synthesized proteins, as well as aiding in the refolding of denatured proteins under cellular stress conditions. It is also involved in the regulation of apoptosis and immune response, making it a target of interest for research in areas such as cancer, neurodegenerative diseases, and cellular stress pathways.
By using the HSPA5 Polyclonal Antibody, researchers can detect and analyze the expression levels of HSPA5 in various cell types, providing valuable insights into its function and potential therapeutic applications. This antibody is an essential tool for studies in cell biology, molecular biology, and disease mechanisms related to cellular stress and protein folding pathways.
Product Name:
BiP/GRP78 Rabbit Polyclonal Antibody
SKU:
CAB11366
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 550-654 of human BiP/GRP78 (NP_005338.1).
Sequence:
EEDK KLKE RIDT RNEL ESYA YSLK NQIG DKEK LGGK LSSE DKET MEKA VEEK IEWL ESHQ DADI EDFK AKKK ELEE IVQP IISK LYGS AGPP PTGE EDTA EKDE L
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers.
Purification Method:
Affinity purification
Gene ID:
3309
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using BiP/GRP78 Rabbit pAb (CAB11366) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
