BiP/GRP78 Rabbit Polyclonal Antibody (CAB0241)
- SKU:
- CAB0241
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Product Name: | BiP/GRP78 Rabbit Polyclonal Antibody |
| SKU: | CAB0241 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human BiP/GRP78 (NP_005338.1). |
| Sequence: | EEDK KLKE RIDT RNEL ESYA YSLK NQIG DKEK LGGK LSSE DKET MEKA VEEK IEWL ESHQ DADI EDFK AKKK ELEE IVQP IISK LYGS AGPP PTGE EDTA E |
| Tested Applications: | WB IHC-P ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 |
| Synonyms: | BIP; GRP78; HEL-S-89n; BiP/GRP78 |
| Positive Sample: | HeLa,Mouse liver,Mouse placenta,Rat liver,Rat lung |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cytoplasm, Endoplasmic reticulum lumen, Melanosome. |
| Calculated MW: | 72kDa |
| Observed MW: | 72kDa |
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers.
| Purification Method: | Affinity purification |
| Gene ID: | 3309 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates, using BiP/GRP78 Rabbit pAb (CAB0241) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s. |
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