The HSPA5 Polyclonal Antibody (CAB0241) offered by AssayGenie is a valuable tool for researchers studying the HSPA5 protein, also known as heat shock protein 70 (HSP70). This antibody, generated in rabbits, has high reactivity with human samples and is ideal for use in Western blot applications. By binding specifically to the HSPA5 protein, this antibody enables precise detection and analysis in various cell types.HSPA5 is a crucial chaperone protein involved in protein folding and quality control in the endoplasmic reticulum. Its role in cellular stress response and protein homeostasis makes it an important target for research in various fields, including cancer, neurodegenerative diseases, and metabolic disorders.
Understanding the function and regulation of HSPA5 is essential for developing novel therapeutic strategies targeting protein folding and cellular stress pathways.With its high specificity and sensitivity, the HSPA5 Polyclonal Antibody (CAB0241) from AssayGenie is a valuable tool for researchers investigating the role of HSPA5 in health and disease. Its application in Western blotting provides reliable data for in-depth analysis of HSPA5 expression levels and cellular localization, supporting advancements in the understanding of protein homeostasis and cellular stress response mechanisms.
Product Name:
BiP/GRP78 Rabbit Polyclonal Antibody
SKU:
CAB0241
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human BiP/GRP78 (NP_005338.1).
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers.
Purification Method:
Affinity purification
Gene ID:
3309
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using BiP/GRP78 Rabbit pAb (CAB0241) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
