Anti-HSP70 Antibody (CAB12948)
- SKU:
- CAB12948
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
Anti-HSP70 Antibody (CAB12948)
The Anti-Hsp70 Polyclonal Antibody (CAB12948) is a valuable tool for researchers studying heat shock protein 70 (Hsp70), a highly conserved chaperone protein involved in cellular stress response. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to Hsp70, researchers can accurately detect and analyze the protein in a variety of cell types.Hsp70 is known for its role in protecting cells from stress-induced damage and promoting cell survival. Research has shown that dysregulation of Hsp70 expression is linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.
Therefore, studying the expression and function of Hsp70 is essential for understanding its potential as a therapeutic target in these conditions.By utilizing the Anti-Hsp70 Polyclonal Antibody (CAB12948), researchers can further investigate the role of Hsp70 in cellular stress response pathways, disease progression, and potential therapeutic interventions. This antibody is a valuable tool for immunology, cancer research, and other fields focused on understanding the intricate mechanisms of cellular stress and disease development.
| Antibody Name: | Anti-HSP70 Antibody |
| Antibody SKU: | CAB12948 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70 (NP_005336.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, BxPC-3, 293T, A-549, Mouse brain, Mouse kidney, Mouse lung, Rat lung |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70 (NP_005336.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KITI TNDK GRLS KEEI ERMV QEAE KYKA EDEV QRER VSAK NALE SYAF NMKS AVED EGLK GKIS EADK KKVL DKCQ EVIS WLDA NTLA EKDE FEHK RKEL E |
| Gene ID: | 3303 |
| Uniprot: | P0DMV8 |
| Cellular Location: | |
| Calculated MW: | 70kDa |
| Observed MW: | 70kDa |
| Synonyms: | HSPA1A, HEL-S-103, HSP70-1, HSP70-1A, HSP70.1, HSP70I, HSP72, HSPA1, HSP70 |
| Background: | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. |
| UniProt Protein Function: | HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70. |
| UniProt Protein Details: | Protein type:Chaperone; Heat shock protein; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 6p21.33 Cellular Component: centriole; centrosome; cytoplasm; cytosol; endoplasmic reticulum; extracellular region; focal adhesion; inclusion body; mitochondrion; nuclear speck; nucleoplasm; nucleus; perinuclear region of cytoplasm; ribonucleoprotein complex; ubiquitin ligase complex; vesicle Molecular Function:ATP binding; ATPase activity; ATPase activity, coupled; cadherin binding; denatured protein binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; histone deacetylase binding; protein binding; protein N-terminus binding; receptor binding; RNA binding; ubiquitin protein ligase binding; unfolded protein binding Biological Process: activation of NF-kappaB transcription factor; ATP metabolic process; chaperone-mediated protein complex assembly; mRNA catabolic process; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of protein ubiquitination; negative regulation of transforming growth factor beta receptor signaling pathway; neutrophil degranulation; positive regulation of interleukin-8 production; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein refolding; protein stabilization; regulation of mRNA stability; regulation of protein ubiquitination; response to unfolded protein |
| NCBI Summary: | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P0DMV8 |
| NCBI GenInfo Identifier: | 825168577 |
| NCBI Gene ID: | 3303 |
| NCBI Accession: | P0DMV8.1 |
| UniProt Secondary Accession: | P0DMV8,P08107, P19790, Q5JQI4, Q5SP17, Q9UQL9, Q9UQM0 B4E3B6, |
| UniProt Related Accession: | P0DMV8 |
| Molecular Weight: | 70kDa |
| NCBI Full Name: | Heat shock 70 kDa protein 1A |
| NCBI Synonym Full Names: | heat shock protein family A (Hsp70) member 1A |
| NCBI Official Symbol: | HSPA1AÂ Â |
| NCBI Official Synonym Symbols: | HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103Â Â |
| NCBI Protein Information: | heat shock 70 kDa protein 1A |
| UniProt Protein Name: | Heat shock 70 kDa protein 1A |
| UniProt Synonym Protein Names: | Heat shock 70 kDa protein 1; HSP70-1 |
| Protein Family: | Heat shock 70 kDa protein |
| UniProt Gene Name: | HSPA1AÂ Â |
 | Western blot analysis of extracts of various cell lines, using HSP70 antibody (CAB12948) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using HSP70 antibody (CAB12948) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using HSP70 antibody (CAB12948) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat testis using HSP70 antibody (CAB12948) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using HSP70 Rabbit pAb (CAB12948) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining. |
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