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HSP70 Rabbit Polyclonal Antibody (CAB0284)

SKU:
CAB0284
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Size:
20uL, 100uL
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:HSP70 Rabbit Polyclonal Antibody
SKU:CAB0284
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70 (NP_005336.3).
Sequence:KITI TNDK GRLS KEEI ERMV QEAE KYKA EDEV QRER VSAK NALE SYAF NMKS AVED EGLK GKIS EADK KKVL DKCQ EVIS WLDA NTLA EKDE FEHK RKEL E
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:HSP70; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1A; HEL-S-103
Positive Sample:Mouse brain,293T,HeLa,A549
Conjugate:Unconjugated
Cellular Localization:blood microparticle, centriole, centrosome, cytoplasm, cytosol, endoplasmic reticulum, extracellular exosome, extracellular region, focal adhesion, mitochondrion, nuclear speck, nucleoplasm, nucleus, perinuclear region of cytoplasm, plasma membrane
Calculated MW:70kDa
Observed MW:70kDa

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Purification Method:Affinity purification
Gene ID:3303
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using HSP70 Rabbit pAb (CAB0284) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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