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HSP27/HSPB1 Polyclonal Antibody (CAB21461)

SKU:
CAB21461
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IF
IP
$155 - $431
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:HSP27/HSPB1 Polyclonal Antibody
SKU:CAB21461
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HSP27/HSPB1 (NP_001531.1).
Sequence:MTER RVPF SLLR GPSW DPFR DWYP HSRL FDQA FGLP RLPE EWSQ WLGG SSWP GYVR PLPP AAIE SPAV AAPA YSRA LSRQ LSSG VSEI RHTA DRWR VSLD VNHF APDE LTVK TKDG VVEI
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102; HSP27/HSPB1
Positive Sample:HepG2,HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus, cytoskeleton, spindle.
Calculated MW:23kDa
Observed MW:Refer to figures

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.

Purification Method:Affinity purification
Gene ID:3315
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using HSP27/HSPB1 Rabbit pAb (CAB21461) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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