The HSF1 Polyclonal Antibody (CAB13765) is a valuable tool for researchers studying Heat Shock Factor 1 (HSF1), a transcription factor involved in cellular stress response and regulation of heat shock proteins. This antibody, produced in rabbits, is highly specific for detecting HSF1 in human samples and has been validated for use in Western blot applications.HSF1 plays a critical role in protecting cells from stress-induced damage by activating the expression of heat shock proteins.
Dysregulation of HSF1 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. By using the HSF1 Polyclonal Antibody, researchers can study the expression and activity of HSF1 in different cell types and conditions, providing valuable insights into its role in cellular homeostasis and disease processes.
Product Name:
HSF1 Rabbit Polyclonal Antibody
SKU:
CAB13765
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 350-529 of human HSF1 (NP_005517.1).
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.
Purification Method:
Affinity purification
Gene ID:
3297
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HSF1 Rabbit pAb (CAB13765) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
