The HSD3B1 Polyclonal Antibody (CAB8035) is a valuable tool for researchers studying HSD3B1, a key enzyme involved in steroid hormone biosynthesis. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the HSD3B1 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for investigations in endocrinology and hormone-related disorders.HSD3B1, also known as 3-beta-hydroxysteroid dehydrogenase, is essential for the production of steroid hormones, including testosterone and estrogen, highlighting its importance in normal physiological processes.
Dysregulation of HSD3B1 activity has been linked to various conditions such as hormone-dependent cancers and disorders of sexual development. Therefore, further research on HSD3B1 is crucial for understanding its role in health and disease, potentially leading to the development of targeted therapies and diagnostic tools.
Product Name:
HSD3B1 Rabbit Polyclonal Antibody
SKU:
CAB8035
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human HSD3B1 (NP_000853.1).
The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.
Purification Method:
Affinity purification
Gene ID:
3283
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HSD3B1 antibody (CAB8035) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
