Anti-HSD17B10 Antibody (CAB5448)

Antibody Name:	Anti-HSD17B10 Antibody
Antibody SKU:	CAB5448
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B10 (NP_004484.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Lovo, 293T, HepG2, BT-474, 22Rv1, SH-SY5Y, Mouse liver, Mouse kidney, Rat heart, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human HSD17B10 (NP_004484.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAA CRSV KGLV AVIT GGAS GLGL ATAE RLVG QGAS AVLL DLPN SGGE AQAK KLGN NCVF APAD VTSE KDVQ TALA LAKG KFGR VDVA VNCA GIAV ASKT YNLK KGQT HTLE DFQR VLDV NLMG TFNV IRLV AGEM GQNE PDQG GQRG VIIN TASV AAFE GQVG QAAY SASK GGIV GMTL PIAR DLAP IGIR VMTI APGL FGTP LLTS LPEK VCNF LASQ VPFP SRLG DPAE YAHL VQAI IENP FLNG EVIR LDGA IRMQ P
Gene ID:	3028
Uniprot:	Q99714
Cellular Location:	Mitochondrion
Calculated MW:	25kDa/26kDa
Observed MW:	26kDa

Synonyms:

HSD17B10, 17b-HSD10, ABAD, CAMR, DUPXp11.22, ERAB, HADH2, HCD2, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, HSD10MD

Background:

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

UniProt Protein Function:	HADH2: Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD). Defects in HSD17B10 are the cause of 2-methyl-3- hydroxybutyryl-CoA dehydrogenase deficiency (MHBD deficiency). MHBD deficiency leads to neurological abnormalities, including psychomotor retardation, and, in virtually all patients, loss of mental and motor skills. Defects in HSD17B10 are the cause of mental retardation syndromic X-linked type 10 (MRXS10). MRXS10 is characterized by mild mental retardation, choreoathetosis and abnormal behavior. A chromosomal microduplication involving HSD17B10 and HUWE1 is the cause of mental retardation X-linked type 17 (MRX17); also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub- average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X- linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non- syndromic X-linked mental retardation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; Oxidoreductase; EC 1.1.1.51; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 1.1.1.35; EC 1.1.1.178 Chromosomal Location of Human Ortholog: Xp11.2 Cellular Component: mitochondrion; endoplasmic reticulum; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; plasma membrane Molecular Function:3(or 17)beta-hydroxysteroid dehydrogenase activity; protein binding; 7-alpha-hydroxysteroid dehydrogenase activity; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity; 3-hydroxyacyl-CoA dehydrogenase activity Biological Process: tRNA processing; lipid metabolic process; branched chain family amino acid catabolic process Disease: Mental Retardation, X-linked, Syndromic 10; 17-beta-hydroxysteroid Dehydrogenase X Deficiency
NCBI Summary:	This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids and steroids, and is a subunit of mitochondrial ribonuclease P, which is involved in tRNA maturation. The protein has been implicated in the development of Alzheimer disease, and mutations in the gene are the cause of 17beta-hydroxysteroid dehydrogenase type 10 (HSD10) deficiency. Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq, Aug 2014]
UniProt Code:	Q99714
NCBI GenInfo Identifier:	2492759
NCBI Gene ID:	3028
NCBI Accession:	Q99714.3
UniProt Secondary Accession:	Q99714,Q5H927, Q6IBS9, Q8TCV9, Q96HD5,
UniProt Related Accession:	Q99714
Molecular Weight:	261
NCBI Full Name:	3-hydroxyacyl-CoA dehydrogenase type-2
NCBI Synonym Full Names:	hydroxysteroid (17-beta) dehydrogenase 10
NCBI Official Symbol:	HSD17B10
NCBI Official Synonym Symbols:	ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; 17b-HSD10; DUPXp11.22
NCBI Protein Information:	3-hydroxyacyl-CoA dehydrogenase type-2; mitochondrial RNase P subunit 2; AB-binding alcohol dehydrogenase; mitochondrial ribonuclease P protein 2; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; short chain type dehydrogenase/reductase XH98G2; amyloid-beta peptide binding alcohol dehydrogenase; short chain L-3-hydroxyacyl-CoA dehydrogenase type 2; short chain dehydrogenase/reductase family 5C, member 1; endoplasmic reticulum-associated amyloid beta-peptide-binding protein
UniProt Protein Name:	3-hydroxyacyl-CoA dehydrogenase type-2
UniProt Synonym Protein Names:	17-beta-hydroxysteroid dehydrogenase 10 (EC:1.1.1.51); 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase (EC:1.1.1.178); 3-hydroxyacyl-CoA dehydrogenase type II; Endoplasmic reticulum-associated amyloid beta-peptide-binding protein; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Short-chain type dehydrogenase/reductase XH98G2; Type II HADH
Protein Family:	3-hydroxyacyl-CoA dehydrogenase
UniProt Gene Name:	HSD17B10
UniProt Entry Name:	HCD2_HUMAN

	Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (CAB5448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of various cell lines, using HSD17B10 antibody (CAB5448) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded Human colon using HSD17B10 Rabbit pAb (CAB5448) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using HSD17B10 Polyclonal Antibody (CAB5448) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
HSD17B10 Antibody (PACO00773)	Human HSD17B10 / ERAB ELISA Kit
HSD17B10 Antibody (PACO09806)	Human 3-hydroxyacyl-CoA dehydrogenase type-2 (HSD17B10) ELISA Kit
	ERAB Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)