The HSD17B1 Polyclonal Antibody (CAB10839) is an essential tool for researchers studying HSD17B1, an enzyme involved in the biosynthesis of various steroid hormones, including estrogen and androgen. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the HSD17B1 protein, this antibody allows for the detection and analysis of HSD17B1 expression in a variety of cell types, making it a valuable asset for studies in endocrinology and hormone-related diseases.
HSD17B1 plays a crucial role in the metabolism and synthesis of steroid hormones, making it an attractive target for research in reproductive biology, hormone-dependent cancers, and metabolic disorders. Understanding the function and regulation of HSD17B1 is essential for unraveling its role in hormone-related diseases and developing targeted therapeutic strategies. With the HSD17B1 Polyclonal Antibody, researchers can delve deeper into the mechanisms underlying hormone biosynthesis and explore new avenues for treating hormone-related disorders.
Product Name:
HSD17B1 Rabbit Polyclonal Antibody
SKU:
CAB10839
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human HSD17B1 (NP_000404.2).
This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3292
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HSD17B1 Rabbit pAb (CAB10839) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
