The HSD11B2 Polyclonal Antibody (CAB8077) is a valuable tool for researchers studying HSD11B2, an enzyme involved in the conversion of cortisone to cortisol in the target tissues of glucocorticoids. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications.HSD11B2 plays a crucial role in regulating the balance of active and inactive glucocorticoids, which are hormones involved in a wide range of physiological processes such as metabolism, inflammation, and stress response.
Dysregulation of HSD11B2 has been linked to various metabolic disorders and inflammatory conditions, making it a potential therapeutic target for these diseases.By using the HSD11B2 Polyclonal Antibody, researchers can accurately detect and quantify the expression of HSD11B2 in different cell types and tissues, providing valuable insights into its role in health and disease. This antibody is a valuable tool for studies in endocrinology, metabolism, and drug development, ultimately contributing to the advancement of medical research and the development of novel therapies.
Product Name:
HSD11B2 Rabbit Polyclonal Antibody
SKU:
CAB8077
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 266-405 of human HSD11B2 (NP_000187.3).
There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.
Purification Method:
Affinity purification
Gene ID:
3291
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HepG2 cells, using HSD11B2 antibody (CAB8077) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 15s.
