The HSCB Polyclonal Antibody (CAB15961) is a valuable tool for researchers studying HSCB, a key player in mitochondrial biology. This antibody, raised in rabbits, has been validated for use in various applications, including Western blot and immunohistochemistry.HSCB, also known as HscB chaperone protein, is essential for the proper assembly of iron-sulfur clusters in proteins involved in electron transport and oxidative phosphorylation. Dysfunction of HSCB can lead to mitochondrial disorders and metabolic diseases. Therefore, studying the role of HSCB is important for understanding mitochondrial function and developing potential treatments for related diseases.
With its high reactivity and specificity for human samples, the HSCB Polyclonal Antibody is an excellent choice for researchers in the fields of biochemistry, cell biology, and molecular biology. By enabling the detection and analysis of HSCB in various cellular contexts, this antibody is a valuable tool for advancing our knowledge of mitochondrial biology and its implications in human health and disease.
Product Name:
HSCB Rabbit Polyclonal Antibody
SKU:
CAB15961
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-235 of human HSCB (NP_741999.3).
This gene encodes a DnaJ-type co-chaperone and member of the heat shock cognate B (HscB) family of proteins. The encoded protein plays a role in the synthesis of iron-sulfur clusters, protein cofactors that are involved in the redox reactions of mitochondrial electron transport and other processes. Cells in which this gene is knocked down exhibit reduced activity of iron-sulfur cluster-dependent enzymes including succinate dehydrogenase and aconitase. The encoded protein may stimulate the ATPase activity of the mitochondrial stress-70 protein. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
150274
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HSCB Rabbit pAb (CAB15961) at 1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
