The HS3ST3A1 Polyclonal Antibody (CAB16474) is a valuable tool for researchers studying HS3ST3A1, an enzyme involved in the modification of heparan sulfate chains. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By specifically binding to the HS3ST3A1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an essential component for studies in the fields of glycobiology and cancer research.HS3ST3A1 plays a crucial role in the regulation of heparan sulfate proteoglycans, which are involved in numerous cellular processes including cell adhesion, growth factor signaling, and inflammation.
Dysregulation of HS3ST3A1 has been implicated in various diseases such as cancer, diabetes, and cardiovascular disorders, highlighting its potential as a therapeutic target. By understanding the function and activity of HS3ST3A1, researchers can gain valuable insights into the mechanisms underlying these diseases and develop novel treatment strategies.
Product Name:
HS3ST3A1 Rabbit Polyclonal Antibody
SKU:
CAB16474
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 44-140 of human HS3ST3A1 (NP_006033.1).
Golgi apparatus membrane, Single-pass type II membrane protein.
Calculated MW:
45kDa
Observed MW:
45kDa
Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It is a type II integral membrane protein and possesses heparan sulfate glucosaminyl 3-O-sulfotransferase activity. The sulfotransferase domain of this enzyme is highly similar to the same domain of heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3B1, and these two enzymes sulfate an identical disaccharide. This gene is widely expressed, with the most abundant expression in liver and placenta.
Purification Method:
Affinity purification
Gene ID:
9955
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from U-251MG cells, using HS3ST3A1 Rabbit pAb (CAB16474) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
