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HRP-SA (Streptavidin Horseradish Peroxidase) Activity ELISA Kit (AEFI01231)

SKU:
AEFI01231
Size:
96T
Reactivity:
Universal
Detection Method:
Direct ELISA
Sensitivity:
14uIU/ml
Range:
28.125-1800uIU/ml(1800uU=6ng)
Detection Wavelength:
OD450
$719

Description

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Product Name:HRP-SA (Streptavidin Horseradish Peroxidase) Activity ELISA Kit
SKU:AEFI01231
Size:96T
Reactivity:Universal
Range:28.125-1800uIU/ml(1800uU=6ng)
Sensitivity:14uIU/ml
Detection Method:Direct ELISA
Detection Wavelength:OD450
Reaction Duration:1 hours
Synonyms:HRP-SA ELISA Kit, Streptavidin Horseradish Peroxidase ELISA Kit

This kit was based on direct enzyme-linked immune-sorbent assay technology. Biotin was pre-coated onto 96-well plates. The

Recovery:

Add a certain amount of SA-HRP into the sample. Calculate the recovery by comparing the measured value with the expected amount of SA-HRP in the sample.

Sample TypeRecovery Range(%)Average(%)
Serum(n=5)88-10196
EDTA Plasma(n=5)92-10498
Heparin Plasma(n=5)87-9995
Linearity:

Dilute the sample with a certain amount of SA-HRP at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type1:21:41:8
Serum(n=5)87-105%87-97%85-100%
EDTA Plasma(n=5)93-100%83-98%85-98%
Heparin Plasma(n=5)88-98%82-94%82-96%
Precision:

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

ItemIntra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (uIU/ml)57.2322289256.58226878
Standard deviation2.3210.4155.572.9512.9350.22
CV(%)4.064.696.235.225.725.72

*Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProtocol
1.Wash the plate twice before adding the standard and sample.
2.Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.
3.Washing: Aspirate and wash plates 5 times.
4.Add 90ul TMB Substrate Solution. Incubate 10-20 minutes at 37°C.
5.Add 50ul Stop Solution. Read at 450nm immediately and calculation.
Sample Type:Quantitative detection of hrp-sa(horseradish peroxidase conjugated streptavidin)
Storage:2-8°C(Sealed), Don't cryopreserve.
Specificity:Specifically binds with SA-HRP , no obvious cross reaction with other analogues.

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