HRP polymer-conjugated Goat Anti-Mouse Anti-Rabbit IgG H (CABS080)
The HRP Polymer Conjugated Goat Anti-Mouse Anti-Rabbit IgG (H-CABs080) is a highly efficient secondary antibody designed for use in immunohistochemistry and immunofluorescence applications. This antibody is produced using goats and is conjugated with horseradish peroxidase (HRP) for enhanced signal amplification.With the ability to detect both mouse and rabbit primary antibodies, this HRP-conjugated secondary antibody offers broad compatibility for various experimental setups.
Its high specificity and sensitivity make it an ideal choice for researchers looking to visualize and quantify target proteins in tissue sections or cell cultures.Whether you are conducting basic research or investigating disease mechanisms, the HRP Polymer Conjugated Goat Anti-Mouse Anti-Rabbit IgG (H-CABs080) can help enhance the accuracy and reliability of your results. Trust in this reliable secondary antibody for your immunological studies.
Product Name:
HRP polymer-conjugated Goat Anti-Mouse Anti-Rabbit IgG H
SKU:
CABS080
Size:
100uL
Isotype:
IgG
Host Species:
Goat
Immunogen:
A synthetic peptide corresponding to a sequence within both Mouse IgG and Rabbbit IgG.
Tested Applications:
WB
Recommended Dilution:
WB,1:500 - 1:2000
Positive Sample:
Mouse IgG,Rabbit IgG
Observed MW:
55kDa
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Goat Anti-Mouse Anti-Rabbit IgG H(HRP polymer) (CABS080) at 1:1000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
