The HRP-Conjugated Rabbit Anti-Chicken IgY/IgG (H+L) Antibody (CABS030) is a valuable tool for researchers looking to study avian antibodies in various applications. This antibody has been raised in rabbits and conjugated with HRP for enhanced detection capabilities.With high reactivity towards chicken IgY/IgG antibodies, this antibody is ideal for use in applications such as ELISA, Western blot, and immunohistochemistry. It enables researchers to detect and analyze chicken antibodies with high specificity and sensitivity, making it a reliable choice for immunoassays and antibody detection studies.
Whether studying avian immune responses, antibody production, or infectious diseases in chickens, the HRP-Conjugated Rabbit Anti-Chicken IgY/IgG (H+L) Antibody (CABS030) offers reliable performance and consistent results. Its versatility and specificity make it a valuable tool for researchers in the fields of immunology, veterinary science, and infectious disease research.
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Chicken IgY using HRP Rabbit Anti-Chicken IgY (IgG) (H+L) (CABS030) at 1:5000 dilution.Lysates/proteins: 50ng - 100ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
