The HRP-conjugated Mouse Anti-Rabbit IgG Light Chain (CAB5061) is a valuable tool for researchers studying immune responses involving CD300A, a key cell surface molecule involved in immune regulation. This antibody, raised in mice, is highly specific for rabbit IgG light chain and is validated for use in a variety of applications, including Western blotting.CD300A, also known as an immune inhibitory receptor, plays a critical role in immune homeostasis by regulating inflammation and inhibiting allergic reactions. By targeting this protein with the HRP-conjugated Mouse Anti-Rabbit IgG Light Chain antibody, researchers can gain valuable insights into immune response modulation in various cell types, making it ideal for studies in immunology and cancer research.
With its high reactivity and specificity, this antibody is a valuable tool for researchers seeking to understand the role of CD300A in diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. By enabling the detection and analysis of CD300A protein expression, this antibody can contribute to the development of new therapies that modulate immune function in these contexts.
Product Name:
HRP-conjugated Mouse anti-Rabbit IgG Light Chain
SKU:
CABS061
Size:
100uL
Isotype:
IgG1,Kappa
Host Species:
Mouse
Immunogen:
Rabbit IgG
Tested Applications:
WBIP
Recommended Dilution:
WB,1:5000 - 1:10000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Positive Sample:
Rabbit control IgG
Conjugate:
HRP
Observed MW:
25kDa(Light chain)
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Clone Number:
AMC0531
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of recombinant Rabbit/Mouse/Rat control IgG Protein using HRP-conjugated Mouse Anti-Rabbit IgG Light Chain (CABS061) at 1:10000 dilution.Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(CABS003) at 1:10000 dilution.Lysates/proteins: 100 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time:Â 90s.
