HRP-conjugated Mouse anti Myc-Tag Monoclonal Antibody (CABE026)
The HRP-Conjugated Mouse Anti-Myc Tag Monoclonal Antibody (CABE026) is a highly specific and sensitive tool for detecting the Myc-tag fusion protein in various experimental settings. This antibody, derived from mouse origin, is specifically designed for use in techniques such as Western blot, ELISA, and immunoprecipitation.The Myc tag is commonly used in recombinant protein studies to aid in the detection, purification, and localization of the target protein. The HRP-conjugated version of this antibody allows for enhanced sensitivity and detection of the Myc-tag fusion protein, making it a valuable tool for researchers working in protein expression and localization studies.
With its high specificity and sensitivity, the HRP-Conjugated Mouse Anti-Myc Tag Monoclonal Antibody (CABE026) is an essential reagent for a wide range of research applications, including cell biology, molecular biology, and protein analysis. Its reliable performance and ease of use make it a valuable asset for any laboratory conducting Myc-tagged protein studies.
Product Name:
HRP-conjugated Mouse anti Myc-Tag Monoclonal Antibody
SKU:
CABE026
Size:
100uL
Isotype:
IgG
Host Species:
Mouse
Reactivity:
Species independent
Immunogen:
A synthetic peptide corresponding to Myc tag.
Sequence:
EQKL ISEE DL
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:2000 - 1:5000
Synonyms:
Myc;Myc tag;Myc-tag
Positive Sample:
293T transfected with NLK Protein
Conjugate:
HRP
Observed MW:
60kDa
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method:
Affinity purification
Clone Number:
AMC0048
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of normal 293T cells and 293T transfected with NLK Protein, using HRP-conjugated Mouse anti Myc-Tag mAb (AE026) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
