HRP-conjugated Mouse anti DDDDK-Tag Monoclonal Antibody (CABE024)
- SKU:
- CABE024
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Description
| Product Name: | HRP-conjugated Mouse anti DDDDK-Tag Monoclonal Antibody |
| SKU: | CABE024 |
| Size: | 100uL |
| Isotype: | IgG |
| Host Species: | Mouse |
| Reactivity: | Species independent |
| Immunogen: | A synthetic peptide corresponding to DDDDK tag. |
| Sequence: | DYKDDDDK |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:6000 |
| Synonyms: | DDDDK;DDDDK tag;DDDDK-tag |
| Positive Sample: | 293T-GSK3B-Flag(C-terminal),293F-IRF1-Flag(3*N-terminal),293T |
| Conjugate: | HRP |
| Observed MW: | 50kDa/46kDa/48kDa |
nan
| Purification Method: | Affinity purification |
| Clone Number: | AMC0382-HRP |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3 |
 | Western blot analysis of lysates from 293F cells transfected with IRF1-Flag protein , using HRP-conjugated Mouse anti DDDDK-Tag mAb (AE024) at 1:5000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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