The HRP-conjugated Goat Anti-Rat IgG H&L (CABS028) is a high-quality secondary antibody designed for use in various immunoassays and research applications. This antibody specifically binds to rat IgG, making it an excellent tool for detecting and quantifying rat antibodies in samples.This antibody is HRP-conjugated, allowing for easy and sensitive detection using methods such as Western blotting, ELISA, and immunohistochemistry. Its high specificity and sensitivity make it a reliable choice for researchers studying rat immune responses, antibody production, and various disease models.
With its easy-to-use format and wide range of applications, the HRP-conjugated Goat Anti-Rat IgG H&L (CABS028) is a valuable tool for researchers in immunology, cancer research, and other fields requiring the detection and quantification of rat antibodies. Trust in the quality and performance of this secondary antibody to enhance your research outcomes.
Product Name:
HRP-conjugated Goat anti-Rat IgG (H+L)
SKU:
CABS028
Size:
100uL
Isotype:
Horseradish peroxidase conjugated IgG
Host Species:
Goat
Immunogen:
Rat IgG
Tested Applications:
WB
Recommended Dilution:
WB,1:5000 - 1:10000
Positive Sample:
Rabbit IgG,Mouse IgG,Rat IgG
Conjugate:
HRP
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat IgG, using HRP Goat Anti-Rat IgG (H+L) (CABS028) at 1:1000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
