The HRP-conjugated Goat Anti-Rabbit IgG (H+L) (CAB-S014) is a highly specific and sensitive secondary antibody designed for use in immunohistochemistry, immunofluorescence, and ELISA applications. The antibody is raised in goats and conjugated to Horseradish Peroxidase (HRP), allowing for signal amplification and detection in various research settings.This secondary antibody is particularly suited for detection of rabbit primary antibodies in multiplex assays, providing researchers with a reliable tool for analyzing protein expression and localization.
Its high affinity for rabbit IgG makes it an ideal choice for experiments requiring high levels of sensitivity and specificity.The HRP-conjugated Goat Anti-Rabbit IgG (H+L) (CAB-S014) offers researchers a cost-effective solution for their immunodetection needs, enabling efficient and accurate analysis of target proteins in a variety of biological samples. Its versatility and ease of use make it a valuable addition to any laboratory conducting research in fields such as immunology, cancer biology, and molecular biology.
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (CABS014) at 1:4000-1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
