The HRP-conjugated Goat Anti-Mouse IgG (H+L) Antibody (CABS003) is a versatile tool for research and diagnostic applications. With its high specificity and sensitivity, this antibody is ideal for detecting and analyzing mouse IgG in various samples. The HRP conjugation allows for easy and efficient detection using techniques such as ELISA, Western blot, and immunohistochemistry.This antibody is specifically designed to target mouse IgG, a crucial component of the immune system that plays a key role in immune responses and antibody production.
By utilizing this antibody, researchers can accurately quantify and study mouse IgG levels in biological samples, providing valuable insights into immune function and disease pathology.Whether you are studying immune responses, antibody production, or autoimmune diseases, the HRP-conjugated Goat Anti-Mouse IgG (H+L) Antibody (CABS003) is a reliable tool to help advance your research. Trust in its performance and accuracy to provide meaningful results for your experiments.
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using GAPDH antibody as the primary antibody.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) antibody (CABS003) at 1:5000/1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
