The HRP-Conjugated Donkey anti-Rabbit IgG (H+L) - CAB035 antibody is a powerful tool for researchers studying immune responses and protein detection. This antibody, raised in donkeys, is specifically designed to target rabbit IgG, making it an excellent choice for experiments involving rabbit primary antibodies. The HRP conjugation allows for easy detection using methods such as Western blotting, immunohistochemistry, and ELISA.With high reactivity and specificity for rabbit IgG, this antibody is ideal for use in a variety of research applications, from basic immunology studies to disease-specific research.
Its reliable performance and ease of use make it a valuable tool for scientists looking to explore the intricate mechanisms of the immune system and protein interactions.Overall, the HRP-Conjugated Donkey anti-Rabbit IgG (H+L) - CAB035 antibody is a versatile and essential reagent for any laboratory conducting research in the fields of immunology, cancer biology, and beyond. Its ability to detect rabbit IgG with precision and sensitivity makes it a valuable asset in the quest for understanding and treating various diseases and disorders.
Product Name:
HRP-conjugated Donkey anti-Rabbit IgG (H+L)
SKU:
CABS038
Size:
100uL
Isotype:
Horseradish peroxidase conjugated IgG
Host Species:
Donkey
Immunogen:
Rabbit IgG
Tested Applications:
WBIHC-P
Recommended Dilution:
WB,1:5000 - 1:100000 IHC-P,1:50 - 1:200
Positive Sample:
HeLa
Conjugate:
HRP
Observed MW:
42kDa
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using β-actin antibody as the primary antibody.Secondary antibody: HRP Donkey Anti-Rabbit IgG (H+L) antibody (CABS038) at 1:2000-1:30000 dilutionLysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
