The HRP-conjugated Donkey Anti-Mouse IgG (H+L) (CABS033) is a highly specific and reliable secondary antibody designed for research involving mouse IgG in various applications. This antibody is conjugated to Horseradish Peroxidase (HRP), allowing for easy and sensitive detection of target proteins in assays such as Western blotting, immunohistochemistry, and ELISA.The HRP-conjugated Donkey Anti-Mouse IgG (H+L) (CABS033) is raised in donkeys and has been validated for its specificity and low background reactivity with mouse IgG.
This secondary antibody is ideal for researchers studying mouse proteins in immunology, cell biology, and molecular biology, providing accurate and reproducible results.With its high specificity and sensitivity, the HRP-conjugated Donkey Anti-Mouse IgG (H+L) (CABS033) is a valuable tool for detecting and quantifying mouse IgG in a variety of experimental settings, making it a versatile and essential reagent for research laboratories.
Product Name:
HRP-conjugated Donkey anti-Mouse IgG (H+L)
SKU:
CABS033
Size:
100uL
Isotype:
Horseradish peroxidase conjugated IgG
Host Species:
Donkey
Immunogen:
Mouse IgG
Tested Applications:
WBIHC-P
Recommended Dilution:
WB,1:10000 - 1:30000 IHC-P,1:500 - 1:1000
Positive Sample:
Mouse control IgG,HeLa
Conjugate:
HRP
Observed MW:
25kDa(Light chain),55kDa(Heavy chain)/36kDa
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.75% BSA,50% glycerol,pH7.3.
Western blot analysis of recombinant Mouse control IgG Protein using HRP Donkey Anti-Mouse IgG (H+L)(CABS033) at 1:10000 dilution.Secondary antibody:HRP Donkey Anti-Mouse IgG (H+L)(CABS033) at 1:10000 dilution.Lysates/proteins: 100ng/50ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
